bira r118g bira entry vector Search Results


mammalian expression plasmid pcdna3 1 bira r118g ha bira  (Addgene inc)
93
Addgene inc mammalian expression plasmid pcdna3 1 bira r118g ha bira
Mammalian Expression Plasmid Pcdna3 1 Bira R118g Ha Bira, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
mammalian expression plasmid pcdna3 1 bira r118g ha bira - by Bioz Stars, 2026-03
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pcdna3 1 mcs bira r118g ha vector  (Addgene inc)
94
Addgene inc pcdna3 1 mcs bira r118g ha vector
Pcdna3 1 Mcs Bira R118g Ha Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 mcs bira r118g ha vector/product/Addgene inc
Average 94 stars, based on 1 article reviews
pcdna3 1 mcs bira r118g ha vector - by Bioz Stars, 2026-03
94/100 stars
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pgwvectorbira r118g ha 53581  (Addgene inc)
93
Addgene inc pgwvectorbira r118g ha 53581
Pgwvectorbira R118g Ha 53581, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgwvectorbira r118g ha 53581/product/Addgene inc
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pgwvectorbira r118g ha 53581 - by Bioz Stars, 2026-03
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plasmid #36047  (OriGene)
90
OriGene plasmid #36047
Plasmid #36047, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid #36047 - by Bioz Stars, 2026-03
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bioid vector  (Addgene inc)
96
Addgene inc bioid vector
A . The cell cycle-specific localization <t>of</t> <t>PCDH7:BirA*-HA</t> in interphase (top panel) and mitosis (bottom panel) cells, fixed and stained with PCDH7 antibody (red) and fluorescence conjugated streptavidin (green), DAPI in blue. B . Western blotting analysis shows the biotinylation efficiency of <t>BioID</t> constructs. Controls; non-transfected, backbone vector-transfected (BirA*-HA and mycBirA*). Samples; PCDH7:BirA*-HA, PCDH7Δcyt:BirA*-HA, myrPalm:BirA*-HA (an unrelated construct as negative control). Upper blot; Cells supplied with biotin. Lower blot; Cells not supplied with biotin. In the absence of biotin, only a basal level of biotinylation was observed for all cell lines (bottom panel). In the presence of biotin, while non-transfected cells showed a basal level of biotinylation, nonspecific biotinylation was observed for the empty BioID vector-transfected cells (top left panel). The inputs (I) obtained from PCDH7 BioID, truncated PCDH7 BioID and MyrPalm BioID transfected cells demonstrated different biotinylation patterns as expected. The biotinylated proteins were successfully enriched at the elute fractions (E) of each sample after streptavidin affinity pulldown (top right panel). C . Western blotting analysis shows the biotinylation specificity of BioID constructs. Upper blot; Cells supplied with biotin. Lower blot; Cells not supplied with biotin. Both PCDH7 and truncated PCDH7 (PCDH7Δcyt) are enriched in the elute fractions (E) after streptavidin pulldown only in the biotin-supplied cells (top panel). No PCDH7 enrichment is observed in the negative control. The full-length PCDH7 was only present in the elute (E) fraction of PCDH7 BioID transfected cells, but not in the truncated PCDH7 BioID transfected cells. PCDH7 was not detected at all in the elute of MyrPalm BioID sample as expected. (top panel). The absence of actin in the elute fractions confirmed the lack of cytosolic contamination (bottom panel). Scale bars: 10 μm
Bioid Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
bioid vector - by Bioz Stars, 2026-03
96/100 stars
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Image Search Results


A . The cell cycle-specific localization of PCDH7:BirA*-HA in interphase (top panel) and mitosis (bottom panel) cells, fixed and stained with PCDH7 antibody (red) and fluorescence conjugated streptavidin (green), DAPI in blue. B . Western blotting analysis shows the biotinylation efficiency of BioID constructs. Controls; non-transfected, backbone vector-transfected (BirA*-HA and mycBirA*). Samples; PCDH7:BirA*-HA, PCDH7Δcyt:BirA*-HA, myrPalm:BirA*-HA (an unrelated construct as negative control). Upper blot; Cells supplied with biotin. Lower blot; Cells not supplied with biotin. In the absence of biotin, only a basal level of biotinylation was observed for all cell lines (bottom panel). In the presence of biotin, while non-transfected cells showed a basal level of biotinylation, nonspecific biotinylation was observed for the empty BioID vector-transfected cells (top left panel). The inputs (I) obtained from PCDH7 BioID, truncated PCDH7 BioID and MyrPalm BioID transfected cells demonstrated different biotinylation patterns as expected. The biotinylated proteins were successfully enriched at the elute fractions (E) of each sample after streptavidin affinity pulldown (top right panel). C . Western blotting analysis shows the biotinylation specificity of BioID constructs. Upper blot; Cells supplied with biotin. Lower blot; Cells not supplied with biotin. Both PCDH7 and truncated PCDH7 (PCDH7Δcyt) are enriched in the elute fractions (E) after streptavidin pulldown only in the biotin-supplied cells (top panel). No PCDH7 enrichment is observed in the negative control. The full-length PCDH7 was only present in the elute (E) fraction of PCDH7 BioID transfected cells, but not in the truncated PCDH7 BioID transfected cells. PCDH7 was not detected at all in the elute of MyrPalm BioID sample as expected. (top panel). The absence of actin in the elute fractions confirmed the lack of cytosolic contamination (bottom panel). Scale bars: 10 μm

Journal: bioRxiv

Article Title: ZDHHC5 directs Protocadherin 7 to the mitotic cell surface and cleavage furrow by a palmitoylation-dependent mechanism for a successful cell division

doi: 10.1101/2020.05.24.111831

Figure Lengend Snippet: A . The cell cycle-specific localization of PCDH7:BirA*-HA in interphase (top panel) and mitosis (bottom panel) cells, fixed and stained with PCDH7 antibody (red) and fluorescence conjugated streptavidin (green), DAPI in blue. B . Western blotting analysis shows the biotinylation efficiency of BioID constructs. Controls; non-transfected, backbone vector-transfected (BirA*-HA and mycBirA*). Samples; PCDH7:BirA*-HA, PCDH7Δcyt:BirA*-HA, myrPalm:BirA*-HA (an unrelated construct as negative control). Upper blot; Cells supplied with biotin. Lower blot; Cells not supplied with biotin. In the absence of biotin, only a basal level of biotinylation was observed for all cell lines (bottom panel). In the presence of biotin, while non-transfected cells showed a basal level of biotinylation, nonspecific biotinylation was observed for the empty BioID vector-transfected cells (top left panel). The inputs (I) obtained from PCDH7 BioID, truncated PCDH7 BioID and MyrPalm BioID transfected cells demonstrated different biotinylation patterns as expected. The biotinylated proteins were successfully enriched at the elute fractions (E) of each sample after streptavidin affinity pulldown (top right panel). C . Western blotting analysis shows the biotinylation specificity of BioID constructs. Upper blot; Cells supplied with biotin. Lower blot; Cells not supplied with biotin. Both PCDH7 and truncated PCDH7 (PCDH7Δcyt) are enriched in the elute fractions (E) after streptavidin pulldown only in the biotin-supplied cells (top panel). No PCDH7 enrichment is observed in the negative control. The full-length PCDH7 was only present in the elute (E) fraction of PCDH7 BioID transfected cells, but not in the truncated PCDH7 BioID transfected cells. PCDH7 was not detected at all in the elute of MyrPalm BioID sample as expected. (top panel). The absence of actin in the elute fractions confirmed the lack of cytosolic contamination (bottom panel). Scale bars: 10 μm

Article Snippet: PCDH7 sequence was amplified from PCDH7:eGFPN1 and cloned into BioID vector (pcDNA3.1 MCS-BirA* (R118G) Addgene; 36047).

Techniques: Staining, Fluorescence, Western Blot, Construct, Transfection, Plasmid Preparation, Negative Control